Centrifuge Instrument instructions
Assembled By :
Nilou Lab
Latest Update:
2020/05/19
Centrifuge Instrument instructions
Purpose
The purpose of developing this guideline is to describe the process of doing work and quality control of centrifugation
2- Scope of application
This instruction applies to different parts of the lab.
3- Responsibility for implementation
Responsible for the implementation of this implementation method with the technical staff of the relevant department and is responsible for quality assurance.
4- Definitions
5- Description of actions
Principles :
Centrifugation is a device that separates materials under the influence of centrifugal force, especially based on differences in mass. There are two types of centrifuges used in the laboratory:
1- Separation of particles from the solution in which they float.
2- Separation of two liquid phases with different densities
Most centrifuges are horizontal hovering (Swinging Bucket or Horizontal head), meaning that the tubes are rotated horizontally, and the position of the tubes is vertical when the router is standing.
Centrifuges that are fixed at an angle or angle head, the tubes are kept in a fixed position of 25-40 degrees relative to the vertical axis of rotation. The rotor can rotate the fixed angle of the sample at a higher speed than the horizontal head rotor.
Centrifugal components:
Centrifuges are a rotor or centrifugal head, a shaft, motor, power switch, timer, speed control, speedometer.
The centrifugal speed per minute does not indicate the force required to separate the two phases, and the term is appropriate for the relative power of the centrifuge (RCF), which is called the relative field of centrifugation.
RCF = 1.118 x 10-5 x r x n2
Radius in centimeters from the center of rotation to the centrifugal tube in the rotor chamber = r
Rotor rotation speed in rpm = n
method:
1- The pipe that you use to put in the centrifuge must have the desired RCF tolerance (polypropylene pipes can normally withstand RCF above 5000 x g).
2- First, check the pipes for cracks or fractures. Avoid centrifuging these pipe tubes.
3- Place the tubes evenly and symmetrically in the centrifuge so that they are balanced. If the rotor is unbalanced, it will cause vibration, centrifugal wear and breakage of the pipes (filling the pipe with water can be used to balance the other side).
Never place the tube in the centrifuge without placing the balance tube (even if the tube is plastic).
4- Close the centrifuge tightly and press the on / off button. Make sure it is locked.
5- Adjust the timer at the desired time and rotate the speed slowly so that the man looks at the speed.
6- After the centrifuge is completely stopped, open the device and slowly remove the pipes.
Control and maintenance:
1- The pipe that is placed in the centrifuge should not be so long that it is out of the box during rotation, because it will cause the pipes to break.
2- Make sure the rotor is completely balanced. (The weight of the racks, pipes and their contents on the opposite side should not differ by more than one percent).
3- The total weight of each rock should not be more than the weight expressed by the manufacturer at the desired speed.
4. Cleanliness of the centrifuge is an important factor in reducing the spread of infectious agents, such as viral hepatitis (especially when breaking pipes). Therefore, every week or after breaking the pipes, you should clean the inside of the clean centrifuge with 0.01% citrimide solution.
Note: In case of breakage or suspicion of breakage of the pipes in the centrifuge, the device should be turned off, wait for 30 minutes, and then proceed to clean and disinfect.
If it turns out that the pipe is broken after turning off the device, close the centrifuge immediately and take the necessary steps after 30 minutes.
Service and repair:
1- Check the speed and telemetry once every three months (calibrate its speed using a tachometer). The difference in allowable speed is 5%.
2- Check the timer once every three months with a calibrator timer. The allowable difference is 10%.
3- Check the centrifugal temperature once every three months. To do this, fill a tube with distilled water and measure the water temperature. Then centrifuge the tubes for 5-10 minutes depending on when it is most used in the desired centrifuge. Then measure the water temperature after centrifugation. The temperature difference should not exceed 5 degrees Celsius.
If any of the above is not acceptable, the centrifuge must be serviced.
Attention:
• At the beginning of each working day, the first person to turn on the centrifuge must complete the Log book, and at the end of each day, the last person to work with the centrifuge will write down the completion time and status in the Log book.
• The temperature control form and timer and centrifuge speed are completed after each control.
Safety considerations for working with the device:
Refer to the topic of control and maintenance.
Purpose
The purpose of developing this guideline is to describe the process of doing work and quality control of centrifugation
2- Scope of application
This instruction applies to different parts of the lab.
3- Responsibility for implementation
Responsible for the implementation of this implementation method with the technical staff of the relevant department and is responsible for quality assurance.
4- Definitions
5- Description of actions
Principles :
Centrifugation is a device that separates materials under the influence of centrifugal force, especially based on differences in mass. There are two types of centrifuges used in the laboratory:
1- Separation of particles from the solution in which they float.
2- Separation of two liquid phases with different densities
Most centrifuges are horizontal hovering (Swinging Bucket or Horizontal head), meaning that the tubes are rotated horizontally, and the position of the tubes is vertical when the router is standing.
Centrifuges that are fixed at an angle or angle head, the tubes are kept in a fixed position of 25-40 degrees relative to the vertical axis of rotation. The rotor can rotate the fixed angle of the sample at a higher speed than the horizontal head rotor.
Centrifugal components:
Centrifuges are a rotor or centrifugal head, a shaft, motor, power switch, timer, speed control, speedometer.
The centrifugal speed per minute does not indicate the force required to separate the two phases, and the term is appropriate for the relative power of the centrifuge (RCF), which is called the relative field of centrifugation.
RCF = 1.118 x 10-5 x r x n2
Radius in centimeters from the center of rotation to the centrifugal tube in the rotor chamber = r
Rotor rotation speed in rpm = n
method:
1- The pipe that you use to put in the centrifuge must have the desired RCF tolerance (polypropylene pipes can normally withstand RCF above 5000 x g).
2- First, check the pipes for cracks or fractures. Avoid centrifuging these pipe tubes.
3- Place the tubes evenly and symmetrically in the centrifuge so that they are balanced. If the rotor is unbalanced, it will cause vibration, centrifugal wear and breakage of the pipes (filling the pipe with water can be used to balance the other side).
Never place the tube in the centrifuge without placing the balance tube (even if the tube is plastic).
4- Close the centrifuge tightly and press the on / off button. Make sure it is locked.
5- Adjust the timer at the desired time and rotate the speed slowly so that the man looks at the speed.
6- After the centrifuge is completely stopped, open the device and slowly remove the pipes.
Control and maintenance:
1- The pipe that is placed in the centrifuge should not be so long that it is out of the box during rotation, because it will cause the pipes to break.
2- Make sure the rotor is completely balanced. (The weight of the racks, pipes and their contents on the opposite side should not differ by more than one percent).
3- The total weight of each rock should not be more than the weight expressed by the manufacturer at the desired speed.
4. Cleanliness of the centrifuge is an important factor in reducing the spread of infectious agents, such as viral hepatitis (especially when breaking pipes). Therefore, every week or after breaking the pipes, you should clean the inside of the clean centrifuge with 0.01% citrimide solution.
Note: In case of breakage or suspicion of breakage of the pipes in the centrifuge, the device should be turned off, wait for 30 minutes, and then proceed to clean and disinfect.
If it turns out that the pipe is broken after turning off the device, close the centrifuge immediately and take the necessary steps after 30 minutes.
Service and repair:
1- Check the speed and telemetry once every three months (calibrate its speed using a tachometer). The difference in allowable speed is 5%.
2- Check the timer once every three months with a calibrator timer. The allowable difference is 10%.
3- Check the centrifugal temperature once every three months. To do this, fill a tube with distilled water and measure the water temperature. Then centrifuge the tubes for 5-10 minutes depending on when it is most used in the desired centrifuge. Then measure the water temperature after centrifugation. The temperature difference should not exceed 5 degrees Celsius.
If any of the above is not acceptable, the centrifuge must be serviced.
Attention:
• At the beginning of each working day, the first person to turn on the centrifuge must complete the Log book, and at the end of each day, the last person to work with the centrifuge will write down the completion time and status in the Log book.
• The temperature control form and timer and centrifuge speed are completed after each control.
Safety considerations for working with the device:
Refer to the topic of control and maintenance.
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